CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: OFYS]

CAT#: AM05207FC-N

CD11a (ITGAL) mouse monoclonal antibody, clone OFYS, FITC

Specifications

Product Data

• Clone Name: OFYS
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Characterization of LFA-1 immunodeficiency.
  Monitoring of subsets in peripheral blood.
  Graft-versus-host disease.
  Analysis of CTL-mediated cytotoxicity.
  Studies of transplantation rejection.
  Functional studies of cell adhesion molecules.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody reacts with LFA-1 alpha.
  This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
• Background: CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion.
• Synonyms: Integrin alpha-L, LFA1, LFA-1
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.