CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: F6.2]

CAT#: AM05208BT-N

CD11b (ITGAM) mouse monoclonal antibody, clone F6.2, Biotin

Specifications

Product Data

• Clone Name: F6.2
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of subsets in peripheral blood.
  Characterization of leukemias and lymphomas.
  Analysis of CTL-mediated cytotoxicity.
  Studies of transplantation rejection.
  Functional studies of cell adhesion molecules.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Formulation: Sterile filtered PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
• Background: (Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.