LEF1 Mouse Monoclonal Antibody [Clone ID: REMB1]

CAT#: AM05260PU-L

LEF1 mouse monoclonal antibody, clone REMB1, Purified

Product Images

Western blot analysis using LEF-1 Antibody (Clone REMB1) on Jurkat cell lysate at 5 ug/ml.

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  Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with LEF-1 Antibody (Clone REMB1) and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1.

Specifications

Product Data

• Clone Name: REMB1
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1-5 µg/ml.
  Immunohistochemistry on Paraffin Sections.
  Immunofluorescence on Paraformaldehyde-Fixed Cells: 1/1000 or 1 µg/ml.
  Positive Control: Jurkat cell lysate.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length recombinant LEF-1 protein.
• Specificity: This antibody recognizes the beta-Catenin binding domain of LEF-1.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.08% Sodium Azide
• Concentration: Lot specific
• Purification: Protein A/G Chromatography
• Database Link:
  Entrez Gene 51176 Human
• Background: Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.
• Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha