Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]

CAT#: AM05265AG-N

Calpain (CAPN1+CAPN2) mouse monoclonal antibody, clone 28F3, Agarose

Specifications

Product Data

• Clone Name: 28F3
• Applications: IP
• Recommended Dilution: ELISA: 0.5-1 ug/ml.
  Western blot: 0.5-1 ug/ml.
  Immunoprecipitation of both the native and denatured protein: 1-2ug/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Calpain purified from human placenta.
• Specificity: This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain.
  React with human and bovine calpains, but does not recognize calpains from rat or mouse.
• Formulation: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as preservative.
  Label: Agarose
  State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
• Concentration: Lot specific
• Purification: Standard chromatographic techniques.
• Conjugation: Agarose
• Background: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
• Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1