Alpha SNAP (NAPA) Mouse Monoclonal Antibody [Clone ID: 16D1]

CAT#: AM05268PU-N

Alpha SNAP (NAPA) mouse monoclonal antibody, clone 16D1, Purified

Product Images

Figure 1. A typical Western Blot (10% SDS-PAGE) of chicken brain (Lane 1), chicken kidney (Lane 2), rat brain (Lane 3) and rat kidney (Lane 4) probed with clone 16D1 at 0.2 mg/ml.

Specifications

Product Data

• Clone Name: 16D1
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/5000.
  Positive Control: Rat brain.
• Reactivities: Bovine, Chicken, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human alpha SNAP.
• Specificity: The antibody recognizes an epitope within the highly conserved domain between amino acids 109 - 193 of SNAP-alpha and SNAP-beta.
• Formulation: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50% Glycerol, pH 7.5
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: Lot specific
• Purification: Standard chromatographic techniques.
• Gene Name: Homo sapiens NSF attachment protein alpha (NAPA), transcript variant 1
• Database Link:
  Entrez Gene 108124 MouseEntrez Gene 140673 RatEntrez Gene 8775 Human
• Background: 38 kDa SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide- sensitive fusion protein (NSF) are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-toendosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. alpha-SNAP (or beta-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
• Synonyms: SNAP-alpha, SNAPA, NAPA