LEF1 (alternate exon) Mouse Monoclonal Antibody [Clone ID: 1C3]

CAT#: AM05275PU-N

LEF1 (alternate exon) mouse monoclonal antibody, clone 1C3, Purified

Product Images

Figure 1. Western blot analysis using LEF-1 (alternate exon) antibody on Cos cells transfected with the indicated proteins.

Specifications

Product Data

• Clone Name: 1C3
• Applications: WB
• Recommended Dilution: Western Blot: Detect LEF-1 protein containing the alternately spliced exon between amino acids 212-241 at 1-5 µg/ml.
  Positive Control: Cos cells transfected with LEF-1 protein.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full-length LEF-1 protein.
• Specificity: This antibody recognizes an epitope in amino acids 227-231 of LEF-1.
  This sequence lies within the context dependent activation domain of LEF-1 and appears to be an alternate splice variant exon of the LEF-1 gene.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: Lot specific
• Database Link:
  Entrez Gene 51176 Human
• Background: Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others.
• Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha