Myog Mouse Monoclonal Antibody [Clone ID: F12B]

CAT#: AM05309PU-N

Myog mouse monoclonal antibody, clone F12B, Purified


USD 490.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Clone Name F12B
Applications ELISA, EMSA, IF, IHC, IP, WB
Recommended Dilution ELISA.
Gel Supershift.
Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 µg/ml).
Immunofluorescence.
Immunoprecipitation (2 µg/ml).
Western Blot (1 µg/ml).
Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Specificity Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
Formulation PBS containing 0.08% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration Lot specific
Gene Name Rattus norvegicus myogenin (Myog)
Background Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Synonyms MYOG, MYF4, Myogenic factor 4, BHLHC3
Reference Data

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