FITC Mouse Monoclonal Antibody [Clone ID: F4/1]

CAT#: AM05332PU-N

FITC mouse monoclonal antibody, clone F4/1, Purified

Specifications

Product Data

• Clone Name: F4/1
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western Blot (1-2 µg/ml).
  Flow Cytometry Staining.
  Immunohistochemistry on Frozen and Formalin/Paraffin tissues (2-4 µg/ml).
  Positive Control: Antibodies labeled with FITC.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
• Specificity: The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein.
  Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversation of fluorescein-labeled detection into immunoenzymatic assays.
• Formulation: PBS containing containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: Lot specific
• Synonyms: Fluorescein Isothiocyanate