III Mouse Monoclonal Antibody [Clone ID: E1]

CAT#: AM05393BT-N

III mouse monoclonal antibody, clone E1, Biotin

Product Images

Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.

Specifications

Product Data

• Clone Name: E1
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot: 1-5 µg/ml.
  Flow Cytometry: 1 µg/10⁶ cells.
  Immunohistochemistry.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
• Specificity: This antibody recognizes M13 bacteriophage (gp3).
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: 1.0 mg/ml
• Purification: Protein A/G Chromatography
• Conjugation: Biotin
• Background: The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
• Synonyms: Attachment protein G3P, Gene 3 protein, Minor coat protein A