Itgax Hamster Monoclonal Antibody [Clone ID: N418]

CAT#: AM05514BT-T

Itgax hamster monoclonal antibody, clone N418, Biotin

Specifications

Product Data

• Clone Name: N418
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/5 - 1/10; use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Mouse spleen dendritic cells.
• Specificity: This antibody recognises the murine homologue of human CD11c, which is a 150/90kD member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
• Formulation: Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide, 1% Bovine Serum Albumin
  Label: Biotin
  State: Liquid purified IgG
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: Biotin
• Database Link:
  Entrez Gene 16411 Mouse
• Background: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
• Synonyms: ITGAX, Integrin alpha-X, Leu M5