SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: 15-414]

CAT#: AM05588FC-N

SIRP alpha (SIRPA) mouse monoclonal antibody, clone 15-414, FITC

Specifications

Product Data

• Clone Name: 15-414
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 1x10e6 cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Monocyte-derived dendritic cells.
• Specificity: This antibody is specific for CD172a, also known as signal-regulatory protein alpha.
• Formulation: Phosphate buffered saline pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Database Link:
  Entrez Gene 140885 Human
• Background: CD172a is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
• Synonyms: SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1