Complement C3 (C3) (Neoantigen, iC3b) Mouse Monoclonal Antibody [Clone ID: 013III-1.16]

CAT#: AM05608PU-N

Complement C3 (C3) (Neoantigen, iC3b) mouse monoclonal antibody, clone 013III-1.16, Purified

Specifications

Product Data

• Clone Name: 013III-1.16
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Histology Positive Control Tissue: Kidney from post streptoccal glomerulonephritis patients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified Human iC3b
• Specificity: AM05608PU-N Recognises 42kDa inactive complement component 3b (iC3b) neoantigen present in blood serum.
• Formulation: Borate buffered saline pH 8.2-8.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 3 (C3)
• Database Link:
  Entrez Gene 718 Human
• Background: Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated.
  iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells.
  iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
• Synonyms: CPAMD1, Complement component 3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus