Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

CAT#: AM05640PU-T

Cd8a rat monoclonal antibody, clone 53-6.7, Purified

Specifications

Product Data

• Clone Name: 53-6.7
• Applications: FC, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunofluorescence.
  Flow Cytometry: Use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: This antibody has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse thymus or spleen.
• Specificity: This antibody detects CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain.
  The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. It has also been used for Depletion of CD8a⁺ cells.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography on Protein G from tissue culture supernatant.
• Database Link:
  Entrez Gene 12525 Mouse
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented. The CD8 alpha chain specifically binds to class I MHC molecules.
• Synonyms: CD8 alpha chain, CD8A, MAL