Annexin V (ANXA5) Mouse Monoclonal Antibody [Clone ID: VAA-33]

CAT#: AM05645FC-N

Annexin V (ANXA5) mouse monoclonal antibody, clone VAA-33, FITC

Specifications

Product Data

• Clone Name: VAA-33
• Applications: FC
• Recommended Dilution: Flow Cytometry: neat
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Annexin V
• Specificity: This antibody specifically recognises Annexin V. Clone VAA-33 has been reported to work in western blotting applications.
• Formulation: Tris Buffered Saline
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.02% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
  Absorption emission: 490 nm / 525 nm
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 308 Human
• Background: AnnexinV is a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.
  Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
• Synonyms: Annexin-5, Annexin V, Lipocortin V, Endonexin II, Calphobindin I, Anchorin CII, ANX5, ENX2, PP4, CBP-I, PAP-I, VAC-alpha