Csf1r Rat Monoclonal Antibody [Clone ID: 604B5 2E11]

CAT#: AM05871RP-N

Csf1r rat monoclonal antibody, clone 604B5 2E11, PE

Product Images

Flow Cytometry: Staining of the mouse J774 cell line with Rat anti Mouse CD115 antibody RPE conjugated (AM05871RP-N)

Specifications

Product Data

• Clone Name: 604B5 2E11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Mouse, Rat
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: RAW 264 cells.
  Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653 myeloma cell line.
• Specificity: This antibody recognizes the CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms.
  This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Phycoerythrin
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Ion Exchange Chromatography
• Conjugation: PE
• Database Link:
  Entrez Gene 12978 Mouse
• Background: CD115, also known as c-fms, macrophage Colony Stimulating Factor Receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain.
  CD115 is expressed by cells of the monocytic lineage and by progenitor cells.
• Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1 receptor