Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

CAT#: AM05879HR-N

Rituximab rat monoclonal antibody, clone MB2 A4, HRP, Purified

Product Images

Detection of Rituximab in direct ELISA: Rituximab was used as the coating reagent followed by rat anti-Rituximab antibody Cat.-No. AM05879HR-N as the detection antibody.

Specifications

Product Data

• Clone Name: MB2 A4
• Applications: ELISA
• Recommended Dilution: ELISA: 1/20000-1/20000.
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: F(ab)2 fragment of Rituximab
• Specificity: This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody.
  This antibody does not recognise other CD20 antibodies.
  It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).
  Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (4,5).
• Formulation: PBS
  Label: HRP
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: HRP stabilizer (containg no Azide, no Mercury, no BSA or other Bovine material, but weak concentrations of Rabbit antibodies).
  Preservative: 0.01% Thiomersal
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: HRP
• Background: Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
• Synonyms: Rituxan, MabThera