CD163 Mouse Monoclonal Antibody [Clone ID: 2A10/11]

CAT#: AM05884FC-N

CD163 mouse monoclonal antibody, clone 2A10/11, FITC

Product Images

Staining of porcine peripheral blood mononuclear cells with FITC conjugated Mouse Anti Porcine CD163 antibody

Specifications

Product Data

• Clone Name: 2A10/11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to 1x10e6 cells in 100 µl.
  Functional assays: Clone is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (6).
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine alveolar macrophages
• Specificity: This antibody recognizes Porcine CD163, a 120kD cell surface glycoprotein that is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified Ig fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein A
• Conjugation: FITC
• Gene Name: -
• Background: CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
• Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker