LAMP1 Mouse Monoclonal Antibody [Clone ID: 4E9/11]

CAT#: AM05888FC-N

LAMP1 mouse monoclonal antibody, clone 4E9/11, FITC

Specifications

Product Data

• Clone Name: 4E9/11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat antibody to label 10e6 cells in 100 µl (Membrane permeabilisation is required).
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine alveolar Macrophages.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
• Specificity: This antibody is specific for Porcine CD107a, a cell surface antigen that is also known as lysosomal-associated membrane protein-1 (LAMP-1).
  CD107a is expressed on macrophages and more weakly on monocytes and granulocytes.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Gene Name: -
• Background: Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
• Synonyms: LAMP-1, LAMPA, LGP120