CD52 Rat Monoclonal Antibody [Clone ID: YTH66.9HL]

CAT#: AM05898PU-N

CD52 rat monoclonal antibody, clone YTH66.9HL, Purified

Specifications

Product Data

• Clone Name: YTH66.9HL
• Applications: CT, FC, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: 1:20.
  Western blot.
  Flow cytometry: Use 10 µl of 1:20 diluted antibody to label 10e6 cells in 100 µl.
  Cytotoxic assays.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes
• Specificity: This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kDa.
• Formulation: Phosphate buffered saline 0.09% Sodium Azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Ammonium sulphate precipitation
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1043 Human
• Background: CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is epressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Synonyms: HE5, CDw52