Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: LP5K]

CAT#: AM05941FC-N

Cytokeratin 7 (KRT7) mouse monoclonal antibody, clone LP5K, FITC, Purified

Specifications

Product Data

• Clone Name: LP5K
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 1x10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/10.
  Immunohistochemistry on Paraffin Sections: 1/10 (Requires protein digestion pre-treatment e.g. trypsin or pronase).
  Histology Positive Control: Human skin.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Cytoskeletal proteins from cultured HeLa cells
• Specificity: The antibody reacts with Cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.
• Formulation: PBS
  Label: FITC
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Purification: Ammonium Sulphate Precipitation followed by Ion Exchange Chromatography
• Conjugation: FITC
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This products is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 3855 Human
• Background: Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.
• Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin