HLA Class II DQ Mouse Monoclonal Antibody [Clone ID: FN81]

CAT#: AM05947BT-N

HLA Class II DQ mouse monoclonal antibody, clone FN81, Biotin

Product Images

AM05947BT-N HLA-DQ antibody staining of Human Tonsil Frozen Section.

Specifications

Product Data

• Clone Name: FN81
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: ≤ 0.05 µg/ml (1/4000).
  Recommended Positive Control: Human tonsil.
  Has been described to work in Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated human B lymphocytes.
  The antigen is HLA-DQ. The epitope has not been further characterized.
• Specificity: This Monoclonal antibody FN81 recognizes the Human HLA-DQ antigen.
  Antigen Distribution on Isolated Cells: The HLA DQ antigen is present on approximately 10% of peripheral blood lymphocytes.
• Formulation: PBS, pH 7.2, with 0.05% Kathon as preservative and 5 mg/ml BSA as stabilizer.
  Label: Biotin
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water (= Stock solution).
• Concentration: 0.2 mg/ml
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C.
  Stock solution or aliquots of stock solution can be kept frozen at -20°C.
  Do not freeze working dilutions.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: HLA-DQ is an HLA class II antigen with homology to murine H-2A. HLA class II antigens are mainly expressed on specialized antigen presenting cells (APCs), dendritic cells, B-cells and macrophages. HLA class II antigen is presented on activated T-cells. HLA class II molecules present exogenously derived antigen to the T-cell receptor (TCR) on CD4+ T lymphocytes. Both chains in HLA-DQ are polymorphic. HLA-DQ is expressed after DR and DP during haemotopoietic progenitor cell development. It is strongly expressed in the thymic cortex.
• Synonyms: MHC Class II DQ