CD59 Mouse Monoclonal Antibody [Clone ID: 1F5]

CAT#: AM06017BT-N

CD59 mouse monoclonal antibody, clone 1F5, Biotin

Product Images

Cell Source: Lymphocytes

Specifications

Product Data

• Clone Name: 1F5
• Applications: FC
• Recommended Dilution: Tissue Distribution by Flow Cytometry Analysis:
  Cell Concentration: 1x10e6 cells per test
  Antibody Concentration Used: 1.0μg/10e6 cells
  Cell Source:
  RBC 100%
  Lymphocytes 80.2%
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mouse pre-B tumour cells (RAW112)
  Donor: Lewis rat spleen
  Fusion Partner: S 194/5. XXO. BU-1
• Specificity: This antibody reacts to CD59.
• Formulation: Biotin conjugated IgG buffered in PBS, 0.02% sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: Biotin
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD59 molecule (CD59), transcript variant 2
• Database Link:
  Entrez Gene 966 Human
• Background: CD59 (also known as HRF20, protectin) is a 20 kDa glycoprotein attached to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It blocks MAC formation in the complement pathway by interfering with C9 membrane insertion and polymerization, but it does not affect the generation of C3 and C5 activation products. CD59 is expressed on several cell types, including leukocytes, endothelial and epithelial cells, erythrocytes, and cells of the central nervous system. CD59 belongs to the Ly6 superfamily of proteins.
• Synonyms: MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Hematopoietic cell lineage