CD14 Mouse Monoclonal Antibody [Clone ID: 5A3]

CAT#: AM06089PU-N

CD14 mouse monoclonal antibody, clone 5A3, Purified

Product Images

Immunohistochemical analysis of paraffin-embedded human lymphnode, showing membrane localization using CD14 antibody Cat.-No AM06089PU-N with DAB staining.

• 

  Flow Cytometric analysis of Human PBMC using CD14 antibody

Specifications

Product Data

• Clone Name: 5A3
• Applications: ELISA, FC, IHC
• Recommended Dilution: ELISA: 1/10000.
  Flow Cytometry: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CD14 expressed in E. Coli.
• Specificity: Recognizes CD14
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.03% Sodium Azide
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
• Background: CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
• Synonyms: CD14