Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: 2B7]

CAT#: AM06108PU-N

Estrogen Receptor 1 (ESR1) mouse monoclonal antibody, clone 2B7, Purified

Product Images

Western blot analysis using ER-alpha mouse mAb against human breast carcinoma tissue lysate.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha mouse mAb showing nuclear expression with DAB staining.

Specifications

Product Data

• Clone Name: 2B7
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.
• Specificity: This antibody recognizes ER-alpha.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0,03% Sodium Azide
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 2099 Human
• Background: The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1