Eph receptor B2 (EPHB2) Mouse Monoclonal Antibody [Clone ID: 2D12C6]

CAT#: AM06225SU-N

Eph receptor B2 (EPHB2) mouse monoclonal antibody, clone 2D12C6, Ascites

Product Images

Western blot analysis using EphB2 antibody Cat.-No AM06225SU-N against truncated EphB2 recombinant protein (Lane 1) and extracellular EphB2 (aa19-476)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).

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  Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 antibody Cat.-No AM06225SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 2D12C6
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
• Specificity: Recognizes EPH receptor B2 (EphB2).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2048 Human
• Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
• Synonyms: Ephrin type-B receptor 2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, NY-REN-47