CD19 Mouse Monoclonal Antibody [Clone ID: 2E2]

CAT#: AM06431SU-N

CD19 mouse monoclonal antibody, clone 2E2, Ascites

Product Images

Immunofluorescence analysis of HL-60 (left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

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  Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 2E2
• Applications: FC, IF
• Recommended Dilution: Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CD19 expressed in E. Coli.
• Specificity: This antibody reacts to CD19.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 61 kDa
• Database Link:
  Entrez Gene 930 Human
• Background: The CD19 antigen (95kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
• Synonyms: Leu-12, B-cell marker