CD3E Mouse Monoclonal Antibody [Clone ID: 4E2]

CAT#: AM06435SU-N

CD3E mouse monoclonal antibody, clone 4E2, Ascites

Product Images

Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.

• 

  Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 4E2
• Applications: ELISA, FC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of CD3E expressed in E. Coli.
• Specificity: This antibody reacts to CD3E.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 23 kDa
• Database Link:
  Entrez Gene 916 Human
• Background: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
• Synonyms: T3/Leu-4