IL1 beta (IL1B) Mouse Monoclonal Antibody [Clone ID: 3A6]

CAT#: AM06692SU-N

IL1 beta (IL1B) mouse monoclonal antibody, clone 3A6, Ascites

Product Images

Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)

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  Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.

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  Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Specifications

Product Data

• Clone Name: 3A6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunocytochemistry: 1/200 - 1/1000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human IL1B expressed in E. Coli.
  
• Specificity: This antibody reacts to IL1B.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 31 kDa
• Database Link:
  Entrez Gene 3553 Human
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Synonyms: IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin