Neurofilament (NEFL) Mouse Monoclonal Antibody [Clone ID: 1H3]

CAT#: AM06714PU-N

Neurofilament (NEFL) mouse monoclonal antibody, clone 1H3, Purified

Product Images

Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)

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  Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.

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  Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 1H3
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human NEFL expressed in E. Coli.
  
• Specificity: This antibody reacts to NEFL.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0,5% BSA
  Preservative: 0.05% sodium azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 62 kDa
• Database Link:
  Entrez Gene 4747 Human
• Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
• Synonyms: NF-L,NEFL, NF68, 68 kDa neurofilament protein