PI 3 Kinase catalytic subunit alpha (PIK3CA) Mouse Monoclonal Antibody [Clone ID: 4F3]

CAT#: AM06736PU-N

PI 3 Kinase catalytic subunit alpha (PIK3CA) mouse monoclonal antibody, clone 4F3, Purified

Product Images

Western blot analysis using PIK3CA antibody against human PIK3CA (AA: 881-1068) recombinant protein (Expected MW is 47.4 kDa)

• 

  Western blot analysis using PIK3CA antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

• 
• 

  Immunofluorescence analysis of HeLa cells using PIK3CA antibody (green). Blue: DRAQ5 fluorescent DNA dye.

• 

  Flow Cytometric analysis of Jurkat cells using PIK3CA antibody (green) and negative control (red).

Show all 5 images

Specifications

Product Data

• Clone Name: 4F3
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human PIK3CA expressed in E. Coli.
• Specificity: Recognizes PIK3CA
• Formulation: PBS
  State: Purified
  State: Liquid purified antibody
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 124 kDa
• Database Link:
  Entrez Gene 5290 Human
• Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
• Synonyms: PIK3CA, EC=2.7.1.153, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, PI3K