JNK1 (MAPK8) Mouse Monoclonal Antibody [Clone ID: 1E5]

CAT#: AM06773PU-N

JNK1 (MAPK8) mouse monoclonal antibody, clone 1E5, Purified

Product Images

Western blot analysis using MAPK8 antibody against human MAPK8 (AA: 227-380) recombinant protein.

• 

  Western blot analysis using MAPK8 antibody against A431 (Lane 1), K562 (Lane 2), HeLa (Lane 3), NIH3T3 (Lane 4), PC-12 (Lane 5), and MCF-7 (Lane 6) cell lysate

• 

  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

• 

  Flow Cytometric analysis of HeLa cells using MAPK8 antibody (green) and negative control (red).

Specifications

Product Data

• Clone Name: 1E5
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human MAPK8 expressed in E. Coli.
• Specificity: Recognizes MAPK8
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% protein
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 48.3 kDa
• Database Link:
  Entrez Gene 5599 Human
• Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8