Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

CAT#: AM08023BT-N

Cd8a rat monoclonal antibody, clone 53-6.7, Biotin


USD 260.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Clone Name 53-6.7
Applications FC, IHC, IP
Recommended Dilution Flow Cytometry: ≤  2 µg/106 cells. (Ref.1,6,7,9-15). 
Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.2-5).
Immunoprecipitation (Ref.1,6).
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Spleen cells or thymocyte membranes
Specificity This 53-6.7 antibody recognizes the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa).
In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Formulation PBS containing 0.09% Sodium Azide as preservative
Label: Biotin
State: Liquid purified Ig fraction
Concentration 0.5 mg/ml
Conjugation Biotin
Background In the Mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells.
Synonyms CD8 alpha chain, CD8A, MAL
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.