Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

CAT#: AM08023BT-N

Cd8a rat monoclonal antibody, clone 53-6.7, Biotin

Specifications

Product Data

• Clone Name: 53-6.7
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: ≤ 2 µg/10⁶ cells. (Ref.1,6,7,9-15).
  Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.2-5).
  Immunoprecipitation (Ref.1,6).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Spleen cells or thymocyte membranes
• Specificity: This 53-6.7 antibody recognizes the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa).
  In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  Label: Biotin
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Conjugation: Biotin
• Database Link:
  Entrez Gene 12525 Mouse
• Background: In the Mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8⁺β^- T cells mature extrathymically, while development of the CD8α⁺β⁺ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8⁺ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α⁺ cells.
• Synonyms: CD8 alpha chain, CD8A, MAL