Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

CAT#: AM08023FC-S

Cd8a rat monoclonal antibody, clone 53-6.7, FITC

Product Images

Immunofluorescent Staining: Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD8a-FITC and anti Mouse CD4-R-PE (clone GK1.5), gated on small lymphocytes and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: 1 ug/10e6 cells

Specifications

Product Data

• Clone Name: 53-6.7
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: < / = 2 µg/10e6 cells. (Ref.8,9)
  Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref. 10)
  Immunoprecipitation. (Ref.8)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa).
  In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 12525 Mouse
• Background: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.
• Synonyms: CD8 alpha chain, CD8A, MAL