CD11b Rat Monoclonal Antibody [Clone ID: 3A33]

CAT#: AM08031AC7-N

CD11b rat monoclonal antibody, clone 3A33, APC-Cy7

Specifications

Product Data

• Clone Name: 3A33
• Applications: FC
• Recommended Dilution: Flow Cytometry: ? 0.3 µg/10⁶ cells (Ref.1).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice. (Ref.1)
• Specificity: This antibody is specific to Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: APC-Cy7
  State: Liquid purified Ig fraction.
  Label: Allophycocyanin-Cyanine 7
• Concentration: 0.1 mg/ml
• Conjugation: APC-Cy7
• Background: CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). (Ref.2-6) Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref.2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process. (Ref.4,11) Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. (Ref.12)
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1