Cd22 Rat Monoclonal Antibody [Clone ID: 2D6]

CAT#: AM08033LE-N

Cd22 rat monoclonal antibody, clone 2D6, Low Endotoxin

Specifications

Product Data

• Clone Name: 2D6
• Applications: FC, FN
• Recommended Dilution: Flow Cytometry.(Ref.2)
  Functional Assay: Blocking of B cell homotypic adhesion. (Ref.2)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody is specific to CD22 (Mr 150 kDa), a B Lymphocyte Adhesion Molecule.
• Formulation: PBS without preservatives.
  State: Low Endotoxin
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Database Link:
  Entrez Gene 12483 Mouse
• Background: CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. (Ref.1,2) CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. (Ref.2-4) Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation. (Ref.6-9)
• Synonyms: SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM