CD45 / LCA Rat Monoclonal Antibody [Clone ID: I3/2.3]

CAT#: AM08044RP-N

CD45 / LCA rat monoclonal antibody, clone I3/2.3, PE

Product Images

Immunofluoscent Staining: BALB/c spleen cells were double-stained with Rat anti-Mouse CD45-PE and Rat anti-Mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 g/10e6 cells.

Specifications

Product Data

• Clone Name: I3/2.3
• Applications: FC
• Recommended Dilution: Flow Cytometry: ≤ 0.2 µg/10⁶ cells. (Ref.4,5)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody recognizes a framework epitope present on all CD45 isoforms. (Ref.3-6)
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent
  Label: PE
  State: Liquid purified Ig fraction
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 while those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200