CD45 / LCA (CD45RB) Rat Monoclonal Antibody [Clone ID: C363.16A]

CAT#: AM08045PU-N

CD45 / LCA (CD45RB) rat monoclonal antibody, clone C363.16A, Purified

Specifications

Product Data

• Clone Name: C363.16A
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry. (Ref.1-3)
  Immunoprecipitation. (Ref.2)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cloned mouse Th2 cell lines. (Ref.1)
• Specificity: This antibody is specific to an exon B-dependent epitope of CD45 glycoprotein.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.
  Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells.
  Function: Phosphatase, T cell development and activation Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200