CD45 / LCA (CD45RB) Rat Monoclonal Antibody [Clone ID: C363.16A]

CAT#: AM08045RP-N

CD45 / LCA (CD45RB) rat monoclonal antibody, clone C363.16A, PE

Product Images

Immunofluoscent Staining: BALB/c spleen cells were double-stained with Rat anti-Mouse CD45RB-PE and Rat anti-Mouse CD8a-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.1 g/10e6 cells.

Specifications

Product Data

• Clone Name: C363.16A
• Applications: FC
• Recommended Dilution: Flow Cytometry. < / = 0.2 µg/10e6 cells. (Ref.1-3)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cloned mouse Th2 cell lines. (Ref.1)
• Specificity: This antibody is specific to an exon B-dependent epitope of CD45 glycoprotein.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Background: CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.
  Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells.
  Function: Phosphatase, T cell development and activation Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200