CD49e / ITGA5 Rat Monoclonal Antibody [Clone ID: 5H10-27]

CAT#: AM08047FC-N

CD49e / ITGA5 rat monoclonal antibody, clone 5H10-27, FITC

Specifications

Product Data

• Clone Name: 5H10-27
• Applications: FC
• Recommended Dilution: Flow Cytometry:. < / = 1 µg/10e6 cells. (Ref.1)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C57BL/6 x A/J F1 mouse mast cell line MC/9. (Ref.1)
• Specificity: This antibody is specific to alpha-5 subunit of the integrin alpha-5 beta-1 Fibronectin Receptor.
  It reacts with the alpha-5 chain (VLA-5) of the CD49e/CD29 heterodimeric Fibronectin Receptor. (Ref.1-5)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
  Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. (Ref.1,2-4,6) Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody. (Ref.3)
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA