Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

CAT#: AM08052FC-N

Cd80 rat monoclonal antibody, clone 1G10, FITC

Product Images

Immunofluorescent Staining with CD80 antibody [1G10] (FITC): P815 cells transfected with CD80 or non-transfected P815 control cells were stained with Rat anti-Mouse CD80 antibody (FITC). Large cells were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 3 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 1G10
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.2,7)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises CD80/B7-1 (Mr. 45-47 kDa).
  This Monoclonal CD80 antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 12519 Mouse
• Background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
• Synonyms: CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1