Thy1 Rat Monoclonal Antibody [Clone ID: G7]

CAT#: AM08054FC-N

Thy1 rat monoclonal antibody, clone G7, FITC

Product Images

Immunofluorescent Staining: BALB/c mesenteric lymph node cells were stained with either Rat IgG2c-FITC (as a Negative Control) or Rat anti-Mouse CD90-FITC. Lymphocytes were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: G7
• Applications: FC
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells. (Ref.1, 6-8)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2c
• Clonality: Monoclonal
• Immunogen: Mouse T cell hybridoma C6/G8
• Specificity: This antibody recognises Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A), Mr 28-32 kDa.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 21838 Mouse
• Background: CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (Ref.1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (Ref.1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (Ref.1,5-7). It has also been reported to promote apoptosis of thymocytes and CTL clones (Ref.4,7) and to mediate adhesion of thymocytes to thymic stroma. (Ref.8)
• Synonyms: Thy-1, THY1, CDw90