Enpep Rat Monoclonal Antibody [Clone ID: FG35.4]

CAT#: AM08057RP-N

Enpep rat monoclonal antibody, clone FG35.4, PE

Product Images

Immunofluorescent Staining: Cells from BALB/c fetal liver were cultured with IL-7-producing stromal cells for 3 days at 37°C, stained with Rat anti-Mouse BP1/6C3-PE, and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.2 ug/10e6 cells.

Specifications

Product Data

• Clone Name: FG35.4
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.12)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody is specific to BP1 (Ly-51/6C3)
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Database Link:
  Entrez Gene 13809 Mouse
• Background: BP1, also known as Ly51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa. (Ref.1,2) Originally identified on the surface of pre-B cell lymphomas (Ref.1,3) the cDNA encoding BP1 has been cloned and found to represent Aminopeptidase A. (Ref.4,5) It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of Mouse tissues known to possess aminopeptidase activity. (Ref.6-8) Subsets of normal bone marrow pre-B and B cells express low levels of BP1, which is rapidly upregulated on pre-B cells in the presence of IL-7. (Ref.9,10)
  The BP1 marker is commonly used to identify B cells in different stages of development. (Ref.11)
• Synonyms: EAP, Aminopeptidase A, ENPEP