Hspa5 Rat Monoclonal Antibody [Clone ID: 76-E6]

CAT#: AM08058BT-N

Hspa5 rat monoclonal antibody, clone 76-E6, Biotin

Product Images

Figure 1. Western Blot using AM08058BT-N antibody. Recombinant hamster BiP mutant HCB1- (Lane 1), Full-length recombinant hamster BiP (Lane 2) and Total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Rat anti-BiP-Biotin followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note: We are grateful to We are grateful to Dr. Linda Hendershot for the recombinant hamster BiP proteins and for helpful suggestions.

Specifications

Product Data

• Clone Name: 76-E6
• Applications: ELISA, FN, WB
• Recommended Dilution: ELISA. (Ref.6)
  Immunoblotting: < / = 10 µg/10e6 cells. (Ref.6,8)
  Studies of Immunoglobulin assembly and secretion. Ref.1,2)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody is specific to BIP (Immunoglobulin heavy chain binding protein, Mr 78 kDa).
  Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP. (Ref.8)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Conjugation: Biotin
• Database Link:
  Entrez Gene 14828 Mouse
• Background: The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78. (Ref.2) While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as Molecular Chaperones which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. (Ref.1-5) With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (µ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. (Ref.7)
• Synonyms: 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, BiP