Klrg1 Hamster Monoclonal Antibody [Clone ID: 2F1]

CAT#: AM08067BT-N

Klrg1 hamster monoclonal antibody, clone 2F1, Biotin

Specifications

Product Data

• Clone Name: 2F1
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1,5)
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Activated NK (A-LAK) cells from B6 (H-2b) mice. (Ref.1)
• Specificity: This antibody reacts with Killer Cell Lectin-like Receptor G1 (KLRG1 formerly known as mouse MAFA or 2F1-Ag).
  It stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). (Ref.1,5)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Conjugation: Biotin
• Database Link:
  Entrez Gene 50928 Mouse
• Background: Killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1 Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. (Ref.1,2) These ITIMs interact with the SH2 domains of protein phosphatases such as SHP1. (Ref.3,4) Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors. (Ref.1)
• Synonyms: KLRG1, CLEC15A, MAFA, MAFAL, Mast cell function-associated antigen, MAFA-like receptor, ITIM-containing receptor MAFA-L