CD103 Hamster Monoclonal Antibody [Clone ID: 2E7]

CAT#: AM08068BT-N

CD103 hamster monoclonal antibody, clone 2E7, Biotin

Product Images

Immunofluorescent Staining: BALB/c mesenteric lymph node cells were first labeled with Hamster Anti-Mouse CD103-Biotin, and then double-stained with streptavidin-FITC and Rat Anti-Mouse CD8a-PE (AM08023RP-N or AM08023RP-S). Small lymphocytes were gated and

Specifications

Product Data

• Clone Name: 2E7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD103 + cells (< / = 1 µg/10e6 cells). (Ref.1)
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Specificity: This antibody is specific to CD103/alpha IEL beta7 integrin complex (Mr. 160 and 115 kDa peptides).
  This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation
  is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (Ref.5)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Conjugation: Biotin
• Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (Ref.1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE