CD45 / LCA (CD45RC) Rat Monoclonal Antibody [Clone ID: C455.1F]

CAT#: AM08070RP-N

CD45 / LCA (CD45RC) rat monoclonal antibody, clone C455.1F, PE

Product Images

Immunofluorescent Staining: Cells from BALB/c spleen were double stained with Rat anti-Mouse CD45RC-PE and Rat anti-Mouse CD8a-FITC (AM08023FC-N or AM08023FC-S), following which small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDI

Specifications

Product Data

• Clone Name: C455.1F
• Applications: FC
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD45RC+ cells (< / = 0.2 µg/10e6 cells).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises an exon C-dependent epitope of CD45 glycoprotein.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Background: The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. (Ref.1-4) The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species (See also products AM08044). (Ref.1-4)The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200