MHC Class II I-Ab Rat Monoclonal Antibody [Clone ID: NIMR-4]

CAT#: AM08075FC-N

MHC Class II I-Ab rat monoclonal antibody, clone NIMR-4, FITC

Product Images

Immunofluorescent Staining: BALB/c spleen cells were stained with Rat Anti-Mouse MHC II-FITC, following which small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 g/10e6 cells.

Specifications

Product Data

• Clone Name: NIMR-4
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: Specific to MHC Class II molecules.
  This antibody reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
• Synonyms: H2-Eb1, H-2 class II histocompatibility antigen I-A beta chain