MHC Class II I-Ek Mouse Monoclonal Antibody [Clone ID: 17-3-3S]

CAT#: AM08077FC-N

MHC Class II I-Ek mouse monoclonal antibody, clone 17-3-3S, FITC

Product Images

Immunofluorescent Staining: AKR spleen cells were double-stained with Mouse Anti-Mouse I-Ek-FITC and Rat Anti-Mouse CD3e-PE (Cat#AM08026RP-N or AM08026RP-S). Lymphocytes were gated using and analyzed by flow cytometry. Amount Used: 1 g/10e6 cells.

Specifications

Product Data

• Clone Name: 17-3-3S
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H mouse skin graft and splenocytes. (Ref.1)
• Specificity: This antibody is An epitope on mouse I-Ek MHC class II alloantigen. It reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype.
  Reactivity with H-2d and H-2p haplotypes has not been observed. (Ref.1)
  Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen. (Ref.2)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC