H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

CAT#: AM08080FC-N

H2-D1 mouse monoclonal antibody, clone 28-14-8, FITC

Product Images

Immunofluorescent Staining: Splenocytes from DBA1/J mice were stained with Mouse anti-Mouse H-2Db-FITC. Small lymphocytes were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 28-14-8
• Applications: FC
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells.
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H.SW mouse splenocytes. (Ref.1,2)
• Specificity: This antibody is specific to an epitope in the alpha 3 domain of H-2D^b. It binds to the alpha 3 domain of H-2D^b in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2L^d, but not K^d or D^d, and with H-2D^q and/or L^q.
  The antibody has been shown to block H-2L^d-specific and H-2L^d-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2L^d-positive target cells by Ly-6G2-positive NK cells. (Ref.12).
  Customer feedback: Clone 28-14-8 does not cross-react with Human cells.
• Formulation: 1.0 ml PBS containing 0.09% Sodium Azide as preservative
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 14964 Mouse
• Background: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8⁺ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)
• Synonyms: H-2D(B), H2-D1