MHC Class I H2 Kd/Dd Mouse Monoclonal Antibody [Clone ID: 34-1-2S]

CAT#: AM08081PU-N

MHC Class I H2 Kd/Dd mouse monoclonal antibody, clone 34-1-2S, Purified

Specifications

Product Data

• Clone Name: 34-1-2S
• Applications: CT, FC, IHC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells (see Ref. 11)
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections, see Ref. 11).
  Immunoprecipitation (see Ref. 9)
  Complement-mediated Cytotoxicity.
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3HalphaBDF1 mouse splenocytes.
• Specificity: This antibody is specific to an epitope in the alpha 3 domain that is common to H-2Kd and H-2Dd. It binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 Microglobulin. (Ref.7,8)
  It cross reacts with the alpha 3 domain of H-2Kb. (Ref.1,2)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5) MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. (Ref.3,6)