MHC Class I H2 Kd/Dd Mouse Monoclonal Antibody [Clone ID: 34-1-2S]

CAT#: AM08081PU-N

MHC Class I H2 Kd/Dd mouse monoclonal antibody, clone 34-1-2S, Purified


USD 350.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Clone Name 34-1-2S
Applications CT, FC, IHC
Recommended Dilution Flow Cytometry: < / = 1 µg/10e6 cells (see Ref. 11)
Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections, see Ref. 11).
Immunoprecipitation (see Ref. 9)
Complement-mediated Cytotoxicity.
Reactivities Mouse
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen C3HalphaBDF1 mouse splenocytes.
Specificity This antibody is specific to an epitope in the alpha 3 domain that is common to H-2Kd and H-2Dd. It binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 Microglobulin. (Ref.7,8)
It cross reacts with the alpha 3 domain of H-2Kb. (Ref.1,2)
Formulation 100 mM Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State: Purified
State: Liquid purified Ig fraction.
Concentration 0.5 mg/ml
Background The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5) MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. (Ref.3,6)
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.